| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Glabralysins, Potential New β-Pore-Forming Toxin Family Members from the Schistosomiasis Vector Snail Biomphalaria glabrata
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| Autores: |
Lassalle, Damien
Tetreau, Guillaume
Pinaud, Silvain
Galinier, Richard
Crickmore, Neil
Gourbal, Benjamain
Duval, David
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| Data: |
2020-01
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| Ano: |
2020
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| Palavras-chave: |
Pore-forming toxin
Cry toxin
Invertebrate
Biomphalaria glabrata
Host
Pathogen
Vector snail
Innate immunity
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| Resumo: |
Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that they are structurally related to a subset of Cry toxins from Bacillus thuringiensis, including Cry23, Cry45, and Cry51. Finally, we investigated their gene expression profiles in snail tissues and demonstrated a mosaic transcription. We highlight the specificity in Glabralysin expression following immune stimulation with bacteria, yeast or trematode parasites. Interestingly, one Glabralysin was found to be expressed in immune-specialized hemocytes, and two others were induced following parasite exposure.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00601/71267/69655.pdf
https://archimer.ifremer.fr/doc/00601/71267/69656.zip
DOI:10.3390/genes11010065
https://archimer.ifremer.fr/doc/00601/71267/
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| Editor: |
MDPI AG
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| Formato: |
application/pdf
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| Fonte: |
Genes (2073-4425) (MDPI AG), 2020-01 , Vol. 11 , N. 1 , P. 65 (17p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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